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Abstract |
Gastric cancer is one of the most common cancers in the world, accounting for 8 of the total cancer cases and resulting in 10 of total mortality from cancer casesworldwide.Over 70 of new cases and deaths occur in developing countries,including Eastern Africa and China.Previous epidemiological investigations indicatedthat smoking, alcohol drinking, and Helicobacter pylori infection, may be the majorrisk factors for gastric cancer.However, only a small proportion of the peopleconstantly exposed to these environmental factors eventually develop gastric cancer,indicating that host genetic factors may have critical functions in gastriccarcinogenesis.<br>A molecular epidemiological hospital based case-control study was thusconducted in this thesis study to investigate the independent roles of single nucleotidepolymorphisms (SNPs) in base excision repair (BER) genes (APE1 and NEIL2),carcinogen metabolism gene (CYP2E1) and tumor suppressor pathway gene (MDM2)in causing gastric cancer.The aim was to evaluate the potential association betweenthe single nucleotide polymorphisms (SNPs) in the mentioned genes and theirsusceptibility to gastric cancer in a Northern Jiangsu population.Also, the NEIL2gene mRNA expression was associated with the studied NEIL2 SNP genotypes, toassess whether the NEIL2 genotypes have influence on the NEIL2 mRNA (henceprotein) expression.Moreover, an SNP genotyping assay based on novel magneticnanoparticles was used to genotype the NEIL2 SNP (rs804270) in order to assess theeffectiveness of nanomaterials in designing new SNP detection assays. |
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