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Author (up) Reissig, E.C.; More, G.; Massone, A.; Uzal, F.A. url  doi
  Title Sarcocystosis in wild red deer (Cervus elaphus) in Patagonia, Argentina Type Journal Article
  Year 2016 Publication Parasitology Research Abbreviated Journal Parasitol Res  
  Volume Issue Pages  
  Keywords Argentinean nonnative ungulate; Red deer; Sarcocystis taeniata; Sequencing; Tem  
  Abstract Sarcocystis spp. are protozoan parasites with a heteroxenous life cycle, which produce cysts in the muscle of herbivorous animals. In these animal species, sarcocystosis is frequently asymptomatic, although it may occur with high prevalence. Seven Sarcocystis spp. have been described in red deer (Cervus elephus). The aim of this study was to determine the prevalence of sarcocystosis, and to perform the morphological and molecular characterization of Sarcocystis spp. found in wild red deer of the Nahuel Huapi National Park (NHNP), Patagonia, Argentina. Full necropsies of 62 red deer killed by hunters in the NHNP and neighboring areas were performed. Samples of heart and skeletal muscle were examined histologically and selected samples were also examined by transmission electron microscopy (TEM), PCR and sequencing. Sarcocystis spp. thin walled cysts were detected in 62 % (38/62) of heart, and in 22 % (3/14) of skeletal muscle samples examined histologically. TEM revealed a smooth and thin cyst wall (</=1 mum), with scarce and separated ribbon-like protrusions. A total of three partial and one full 18S ribosomal RNA (rRNA) gene sequences were obtained, and showed the highest identity (>/=99 %) with Sarcocystis taeniata, a species described in moose (Alces alces). The morphological and molecular results indicate that red deer in Argentina are frequently infected with S. taeniata, a species for which the definitive host is unknown. The present results also confirm that Sarcocystis spp. using cervids as intermediate host are not host-specific. Further studies are needed to improve the epidemiological knowledge of Sarcocystosis in red deer.  
  Address California Animal Health & Food Safety Laboratory System, San Bernardino Branch, School of Veterinary Medicine, University of California, Davis, 105 W Central Ave, San Bernardino, CA, 92408, USA  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0932-0113 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:26779923 Approved no  
  Call Number ref @ user @ Serial 92322  
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