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Author (up) Burbulla, L.F.; Beaumont, K.G.; Mrksich, M.; Krainc, D. url  doi
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  Title Micropatterning Facilitates the Long-Term Growth and Analysis of iPSC-Derived Individual Human Neurons and Neuronal Networks Type Journal Article
  Year 2016 Publication Advanced Healthcare Materials Abbreviated Journal Adv Healthc Mater  
  Volume 5 Issue 15 Pages 1894-1903  
  Keywords aging; human induced pluripotent stem cells; long-term neuronal culture; microcontact printing technology; neurodegenerative disorders  
  Abstract The discovery of induced pluripotent stem cells (iPSCs) and their application to patient-specific disease models offers new opportunities for studying the pathophysiology of neurological disorders. However, current methods for culturing iPSC-derived neuronal cells result in clustering of neurons, which precludes the analysis of individual neurons and defined neuronal networks. To address this challenge, cultures of human neurons on micropatterned surfaces are developed that promote neuronal survival over extended periods of time. This approach facilitates studies of neuronal development, cellular trafficking, and related mechanisms that require assessment of individual neurons and specific network connections. Importantly, micropatterns support the long-term stability of cultured neurons, which enables time-dependent analysis of cellular processes in living neurons. The approach described in this paper allows mechanistic studies of human neurons, both in terms of normal neuronal development and function, as well as time-dependent pathological processes, and provides a platform for testing of new therapeutics in neuropsychiatric disorders.  
  Address Department of Neurology, Northwestern University Feinberg School of Medicine, Chicago, IL, 60611, USA  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 2192-2640 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:27108930 Approved no  
  Call Number ref @ user @ Serial 95960  
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