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Author (up) Meltzer, S.J.; Zhou, D.; Weinstein, W.M.
Title Tissue-specific expression of c-Ha-ras in premalignant gastrointestinal mucosae Type Journal Article
Year 1989 Publication Experimental and Molecular Pathology Abbreviated Journal Exp Mol Pathol
Volume 51 Issue 3 Pages 264-274
Keywords Adenomatous Polyposis Coli/*genetics; Barrett Esophagus/*genetics; Colitis, Ulcerative/*genetics; Colon/physiology; Gene Amplification; Humans; Intestinal Mucosa/pathology/*physiology; Methylation; Oncogene Protein p21(ras)/*genetics; Oncogene Proteins/genetics; Phosphoproteins/genetics; Precancerous Conditions/genetics; Proto-Oncogene Proteins/genetics; Proto-Oncogene Proteins c-myc; *Proto-Oncogenes; Tumor Suppressor Protein p53
Abstract The molecular mechanisms underlying premalignant gastrointestinal diseases, such as ulcerative colitis and Barrett's esophagus, remain unknown. For this reason, the expression of the protooncogene c-Ha-ras was studied in ulcerative colitis and Barrett's esophagus. Total cellular RNA was extracted from different regions of the gastrointestinal tract in these two diseases. Expression of c-Ha-ras was greater in proximal than in distal colon and undetectable in Barrett's esophagus. These regional differences in expression were not seen with the control gene beta-actin or with the protooncogenes c-myc and p53. In order to evaluate structural factors contributing to expression, amplification and methylation of c-Ha-ras DNA were studied in these tissues by Southern and slot blotting. No amplification of c-Ha-ras or six other protooncogenes was detected. These data suggest tissue-specific regulation of c-Ha-ras expression in the gastrointestinal tract in certain premalignant disease states.
Address Department of Medicine, University of Maryland, Baltimore
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0014-4800 ISBN Medium
Area Expedition Conference
Notes PMID:2557232 Approved no
Call Number ref @ user @ Serial 96511
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