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Author |
Meltzer, S.J.; Zhou, D.; Weinstein, W.M. |
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Title  |
Tissue-specific expression of c-Ha-ras in premalignant gastrointestinal mucosae |
Type |
Journal Article |
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Year |
1989 |
Publication |
Experimental and Molecular Pathology |
Abbreviated Journal |
Exp Mol Pathol |
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Volume |
51 |
Issue |
3 |
Pages |
264-274 |
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Keywords |
Adenomatous Polyposis Coli/*genetics; Barrett Esophagus/*genetics; Colitis, Ulcerative/*genetics; Colon/physiology; Gene Amplification; Humans; Intestinal Mucosa/pathology/*physiology; Methylation; Oncogene Protein p21(ras)/*genetics; Oncogene Proteins/genetics; Phosphoproteins/genetics; Precancerous Conditions/genetics; Proto-Oncogene Proteins/genetics; Proto-Oncogene Proteins c-myc; *Proto-Oncogenes; Tumor Suppressor Protein p53 |
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Abstract |
The molecular mechanisms underlying premalignant gastrointestinal diseases, such as ulcerative colitis and Barrett's esophagus, remain unknown. For this reason, the expression of the protooncogene c-Ha-ras was studied in ulcerative colitis and Barrett's esophagus. Total cellular RNA was extracted from different regions of the gastrointestinal tract in these two diseases. Expression of c-Ha-ras was greater in proximal than in distal colon and undetectable in Barrett's esophagus. These regional differences in expression were not seen with the control gene beta-actin or with the protooncogenes c-myc and p53. In order to evaluate structural factors contributing to expression, amplification and methylation of c-Ha-ras DNA were studied in these tissues by Southern and slot blotting. No amplification of c-Ha-ras or six other protooncogenes was detected. These data suggest tissue-specific regulation of c-Ha-ras expression in the gastrointestinal tract in certain premalignant disease states. |
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Address |
Department of Medicine, University of Maryland, Baltimore |
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Language |
English |
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ISSN |
0014-4800 |
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Notes |
PMID:2557232 |
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no |
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Call Number |
ref @ user @ |
Serial |
96511 |
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