toggle visibility Search & Display Options

Select All    Deselect All
 |   | 
Details
   print
  Records Links
Author (up) Munthe, S.; Halle, B.; Boldt, H.B.; Christiansen, H.; Schmidt, S.; Kaimal, V.; Xu, J.; Zabludoff, S.; Mollenhauer, J.; Poulsen, F.R.; Kristensen, B.W. url  doi
openurl 
  Title Shift of microRNA profile upon glioma cell migration using patient-derived spheroids and serum-free conditions Type Journal Article
  Year 2017 Publication Journal of Neuro-Oncology Abbreviated Journal J Neurooncol  
  Volume 132 Issue 1 Pages 45-54  
  Keywords Glioblastoma; MicroRNA; Migration; Serum-free; Target  
  Abstract Glioblastoma multiforme (GBM) is the most frequent malignant primary brain tumor. A major reason for the overall median survival being only 14.6 months is migrating tumor cells left behind after surgery. Another major reason is tumor cells having a so-called cancer stem cell phenotype being therefore resistant towards traditional chemo- and radiotherapy. A group of novel molecular targets are microRNAs (miRNAs). MiRNAs are small non-coding RNAs exerting post-transcriptional regulation of gene expression. The aim of this study was to identify differentially expressed miRNAs in migrating GBM cells using serum-free stem cell conditions. We used patient-derived GBM spheroid cultures for a novel serum-free migration assay. MiRNA expression of migrating tumor cells isolated at maximum migration speed was compared with corresponding spheroids using an OpenArray Real-Time PCR System. The miRNA profiling revealed 30 miRNAs to be differentially expressed. In total 13 miRNAs were upregulated and 17 downregulated in migrating cells compared to corresponding spheroids. The three most deregulated miRNAs, miR-1227 (up-regulated), miR-32 (down-regulated) and miR-222 (down-regulated), were experimentally overexpressed. A non-significantly increased migration rate was observed after miR-1227 overexpression. A significantly reduced migration rate was observed after miR-32 and miR-222 overexpression. In conclusion a shift in microRNA profile upon glioma cell migration was identified using an assay avoiding serum-induced migration. Both the miRNA profiling and the functional validation suggested that miR-1227 may be associated with increased migration and miR-32 and miR-222 with decreased migration. These miRNAs may represent potential novel targets in migrating glioma cells.  
  Address Institute of Clinical Research, University of Southern Denmark, Winslowparken 19, 5000, Odense C, Denmark. bjarne.winther.kristensen@rsyd.dk  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0167-594X ISBN Medium  
  Area Expedition Conference  
  Notes PMID:28091986 Approved no  
  Call Number ref @ user @ Serial 96611  
Permanent link to this record
 

 
Author (up) Navarrete-Reyes, A.P.; Medina-Rimoldi, C.T.; Avila-Funes, J.A. url  doi
openurl 
  Title Correlates of subjective transportation deficiency among older adults attending outpatient clinics in a tertiary care hospital in Mexico City Type Journal Article
  Year 2017 Publication Geriatrics & Gerontology International Abbreviated Journal Geriatr Gerontol Int  
  Volume Issue Pages  
  Keywords Latin America; disability; mobility; older adults; transportation  
  Abstract AIM: Older adults frequently report problems of transportation. Little is known about the correlates of transportation deficiency in Latin America. Therefore, the aim of the present study was to determine the correlates of subjective transportation deficiency (STD) among community-dwelling older adults attending a tertiary care hospital in Mexico City. METHODS: Cross-sectional study of 228 participants aged >/=70 years being followed in any of the outpatient clinics of a tertiary care hospital in Mexico City. Data were obtained through a structured questionnaire. Univariate and multivariate logistic regression analyses were carried out in order to identify the correlates of STD. RESULTS: The mean age of the participants was 79.8 years (SD 6.4) and 67.1% were women. STD was present in 46% of participants. The multivariate logistic regression model showed that female sex, illiteracy, mobility disability and the use of an assistive walking device had an independent and statistically significant association with STD. CONCLUSIONS: Female sex, illiteracy, mobility disability and the use of an assistive walking device were independent correlates of STD in the present study. Identifying the frequency and correlates of transportation deficiency in vulnerable populations will allow for the identification and implementation of useful public policies, as well as for the optimization of prevention and treatment strategies in an attempt to preserve mobility and autonomy, especially in low- and middle-income countries where previous work on transportation deficiency is lacking. Geriatr Gerontol Int 2016; : -**.  
  Address Research Center INSERM, Bordeaux, France  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1447-0594 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:28190303 Approved no  
  Call Number ref @ user @ Serial 97045  
Permanent link to this record
 

 
Author (up) Navarrete-Reyes, A.P.; Medina-Rimoldi, C.T.; Avila-Funes, J.A. url  doi
openurl 
  Title Correlates of subjective transportation deficiency among older adults attending outpatient clinics in a tertiary care hospital in Mexico City Type Journal Article
  Year 2017 Publication Geriatrics & Gerontology International Abbreviated Journal Geriatr Gerontol Int  
  Volume Issue Pages  
  Keywords Latin America; disability; mobility; older adults; transportation  
  Abstract AIM: Older adults frequently report problems of transportation. Little is known about the correlates of transportation deficiency in Latin America. Therefore, the aim of the present study was to determine the correlates of subjective transportation deficiency (STD) among community-dwelling older adults attending a tertiary care hospital in Mexico City. METHODS: Cross-sectional study of 228 participants aged >/=70 years being followed in any of the outpatient clinics of a tertiary care hospital in Mexico City. Data were obtained through a structured questionnaire. Univariate and multivariate logistic regression analyses were carried out in order to identify the correlates of STD. RESULTS: The mean age of the participants was 79.8 years (SD 6.4) and 67.1% were women. STD was present in 46% of participants. The multivariate logistic regression model showed that female sex, illiteracy, mobility disability and the use of an assistive walking device had an independent and statistically significant association with STD. CONCLUSIONS: Female sex, illiteracy, mobility disability and the use of an assistive walking device were independent correlates of STD in the present study. Identifying the frequency and correlates of transportation deficiency in vulnerable populations will allow for the identification and implementation of useful public policies, as well as for the optimization of prevention and treatment strategies in an attempt to preserve mobility and autonomy, especially in low- and middle-income countries where previous work on transportation deficiency is lacking. Geriatr Gerontol Int 2016; : -**.  
  Address Research Center INSERM, Bordeaux, France  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1447-0594 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:28190303 Approved no  
  Call Number ref @ user @ Serial 97085  
Permanent link to this record
 

 
Author (up) Navarrete-Reyes, A.P.; Medina-Rimoldi, C.T.; Avila-Funes, J.A. url  doi
openurl 
  Title Correlates of subjective transportation deficiency among older adults attending outpatient clinics in a tertiary care hospital in Mexico City Type Journal Article
  Year 2017 Publication Geriatrics & Gerontology International Abbreviated Journal Geriatr Gerontol Int  
  Volume Issue Pages  
  Keywords Latin America; disability; mobility; older adults; transportation  
  Abstract AIM: Older adults frequently report problems of transportation. Little is known about the correlates of transportation deficiency in Latin America. Therefore, the aim of the present study was to determine the correlates of subjective transportation deficiency (STD) among community-dwelling older adults attending a tertiary care hospital in Mexico City. METHODS: Cross-sectional study of 228 participants aged >/=70 years being followed in any of the outpatient clinics of a tertiary care hospital in Mexico City. Data were obtained through a structured questionnaire. Univariate and multivariate logistic regression analyses were carried out in order to identify the correlates of STD. RESULTS: The mean age of the participants was 79.8 years (SD 6.4) and 67.1% were women. STD was present in 46% of participants. The multivariate logistic regression model showed that female sex, illiteracy, mobility disability and the use of an assistive walking device had an independent and statistically significant association with STD. CONCLUSIONS: Female sex, illiteracy, mobility disability and the use of an assistive walking device were independent correlates of STD in the present study. Identifying the frequency and correlates of transportation deficiency in vulnerable populations will allow for the identification and implementation of useful public policies, as well as for the optimization of prevention and treatment strategies in an attempt to preserve mobility and autonomy, especially in low- and middle-income countries where previous work on transportation deficiency is lacking. Geriatr Gerontol Int 2016; : -**.  
  Address Research Center INSERM, Bordeaux, France  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1447-0594 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:28190303 Approved no  
  Call Number ref @ user @ Serial 97125  
Permanent link to this record
 

 
Author (up) Navarrete-Reyes, A.P.; Medina-Rimoldi, C.T.; Avila-Funes, J.A. url  doi
openurl 
  Title Correlates of subjective transportation deficiency among older adults attending outpatient clinics in a tertiary care hospital in Mexico City Type Journal Article
  Year 2017 Publication Geriatrics & Gerontology International Abbreviated Journal Geriatr Gerontol Int  
  Volume Issue Pages  
  Keywords Latin America; disability; mobility; older adults; transportation  
  Abstract AIM: Older adults frequently report problems of transportation. Little is known about the correlates of transportation deficiency in Latin America. Therefore, the aim of the present study was to determine the correlates of subjective transportation deficiency (STD) among community-dwelling older adults attending a tertiary care hospital in Mexico City. METHODS: Cross-sectional study of 228 participants aged >/=70 years being followed in any of the outpatient clinics of a tertiary care hospital in Mexico City. Data were obtained through a structured questionnaire. Univariate and multivariate logistic regression analyses were carried out in order to identify the correlates of STD. RESULTS: The mean age of the participants was 79.8 years (SD 6.4) and 67.1% were women. STD was present in 46% of participants. The multivariate logistic regression model showed that female sex, illiteracy, mobility disability and the use of an assistive walking device had an independent and statistically significant association with STD. CONCLUSIONS: Female sex, illiteracy, mobility disability and the use of an assistive walking device were independent correlates of STD in the present study. Identifying the frequency and correlates of transportation deficiency in vulnerable populations will allow for the identification and implementation of useful public policies, as well as for the optimization of prevention and treatment strategies in an attempt to preserve mobility and autonomy, especially in low- and middle-income countries where previous work on transportation deficiency is lacking. Geriatr Gerontol Int 2016; : -**.  
  Address Research Center INSERM, Bordeaux, France  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 1447-0594 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:28190303 Approved no  
  Call Number ref @ user @ Serial 97146  
Permanent link to this record
Select All    Deselect All
 |   | 
Details
   print

Save Citations:
Export Records: