TY - JOUR AU - Zapotoczna, M. AU - Forde, E. AU - Hogan, S. AU - Humphreys, H. AU - O'Gara, J.P. AU - Fitzgerald-Hughes, D. AU - Devocelle, M. AU - O'Neill, E. PY - 2017// TI - Eradication of Staphylococcus aureus Biofilm Infections Using Synthetic Antimicrobial Peptides T2 - J Infect Dis JO - The Journal of Infectious Diseases SP - 975 EP - 983 VL - 215 IS - 6 KW - Animals KW - Anti-Bacterial Agents/*pharmacology KW - Biofilms/*drug effects KW - Catheter-Related Infections/*drug therapy KW - Cytokines/blood KW - Disease Models KW - Animal KW - Humans KW - Methicillin-Resistant Staphylococcus aureus/*drug effects KW - Microbial Sensitivity Tests KW - Peptides/*pharmacology KW - Peptides KW - Cyclic/pharmacology KW - Rats KW - Sprague-Dawley KW - Staphylococcal Infections/*drug therapy KW - Vancomycin/administration & KW - dosage KW - *Staphylococcus aureus KW - *antimicrobial peptides (AMPs) KW - *biofilm KW - *catheter lock solution (CLS) AB - Here, we demonstrate that antimicrobial peptides (AMPs) are an effective antibiofilm treatment when applied as catheter lock solutions (CLSs) against S. aureus biofilm infections. The activity of synthetic AMPs (Bac8c, HB43, P18, Omiganan, WMR, Ranalexin, and Polyphemusin) was measured against early and mature biofilms produced by methicillin-resistant S. aureus and methicillin-susceptible S. aureus isolates from patients with device-related infections grown under in vivo-relevant biofilm conditions. The cytotoxic and hemolytic activities of the AMPs against human cells and their immunomodulatory potential in human blood were also characterized. The D-Bac8c2,5Leu variant emerged as the most effective AMP during in vitro studies and was also highly effective in eradicating S. aureus biofilm infection when used in a CLS rat central venous catheter infection model. These data support the potential use of D-Bac8c2,5Leu, alone or in combination with other AMPs, in the treatment of S. aureus intravenous catheter infections. SN - 0022-1899 UR - http://www.ncbi.nlm.nih.gov/pubmed/28453851 UR - PM:28453851 N1 - PMID:28453851 ID - Zapotoczna_etal2017 ER -