TY  - JOUR
AU  - Zapotoczna, M.
AU  - Forde, E.
AU  - Hogan, S.
AU  - Humphreys, H.
AU  - O'Gara, J.P.
AU  - Fitzgerald-Hughes, D.
AU  - Devocelle, M.
AU  - O'Neill, E.
PY  - 2017//
TI  - Eradication of Staphylococcus aureus Biofilm Infections Using Synthetic Antimicrobial Peptides
T2  - J Infect Dis
JO  - The Journal of Infectious Diseases
SP  - 975
EP  - 983
VL  - 215
IS  - 6
KW  - Animals
KW  - Anti-Bacterial Agents/*pharmacology
KW  - Biofilms/*drug effects
KW  - Catheter-Related Infections/*drug therapy
KW  - Cytokines/blood
KW  - Disease Models
KW  - Animal
KW  - Humans
KW  - Methicillin-Resistant Staphylococcus aureus/*drug effects
KW  - Microbial Sensitivity Tests
KW  - Peptides/*pharmacology
KW  - Peptides
KW  - Cyclic/pharmacology
KW  - Rats
KW  - Sprague-Dawley
KW  - Staphylococcal Infections/*drug therapy
KW  - Vancomycin/administration &amp
KW  - dosage
KW  - *Staphylococcus aureus
KW  - *antimicrobial peptides (AMPs)
KW  - *biofilm
KW  - *catheter lock solution (CLS)
AB  - Here, we demonstrate that antimicrobial peptides (AMPs) are an effective antibiofilm treatment when applied as catheter lock solutions (CLSs) against S. aureus biofilm infections. The activity of synthetic AMPs (Bac8c, HB43, P18, Omiganan, WMR, Ranalexin, and Polyphemusin) was measured against early and mature biofilms produced by methicillin-resistant S. aureus and methicillin-susceptible S. aureus isolates from patients with device-related infections grown under in vivo-relevant biofilm conditions. The cytotoxic and hemolytic activities of the AMPs against human cells and their immunomodulatory potential in human blood were also characterized. The D-Bac8c2,5Leu variant emerged as the most effective AMP during in vitro studies and was also highly effective in eradicating S. aureus biofilm infection when used in a CLS rat central venous catheter infection model. These data support the potential use of D-Bac8c2,5Leu, alone or in combination with other AMPs, in the treatment of S. aureus intravenous catheter infections.
SN  - 0022-1899
UR  - http://www.ncbi.nlm.nih.gov/pubmed/28453851
UR  - PM:28453851
N1  - PMID:28453851
ID  - Zapotoczna_etal2017
ER  -