TY  - JOUR
AU  - Kim, M.Y.
AU  - Park, S.-J.
AU  - Shim, J.W.
AU  - Song, Y.J.
AU  - Yang, K.
AU  - Heo, K.
PY  - 2017//
TI  - Accumulation of low-dose BIX01294 promotes metastatic potential of U251 glioblastoma cells
T2  - Oncol Lett
JO  - Oncology Letters
SP  - 1767
EP  - 1774
VL  - 13
IS  - 3
KW  - Bix01294
KW  - epithelial-mesenchymal transition
KW  - glioblastoma stem cells
KW  - metastasis
AB  - BIX01294 (Bix) is known to be a euchromatic histone-lysine N-methyltransferase 2 inhibitor and treatment with Bix suppresses cancer cell survival and proliferation. In the present study, it was observed that sequential treatment with low-dose Bix notably increases glioblastoma cell migration and metastasis. It was demonstrated that U251 cells sequentially treated with low-dose Bix exhibited induced characteristic changes in critical epithelial-mesenchymal transition (EMT) markers, including E-cadherin, N-cadherin, beta-catenin and zinc finger protein SNAI2. Notably, sequential treatment with Bix also increased the expression of cancer stem cell-associated markers, including sex determining region Y-box 2, octamer-binding transcription factor 4 and cluster of differentiation 133. Neurosphere formation was significantly enhanced in cells sequentially treated with Bix, compared with control cells (control: P=0.011; single treatment of Bix, P=0.045). The results of the present study suggest that accumulation of low-dose Bix enhanced the migration and metastatic potential of glioblastoma cells by regulating EMT-associated gene expression, which may be the cause of the altered properties of glioblastoma stem cells.
SN  - 1792-1074
UR  - http://www.ncbi.nlm.nih.gov/pubmed/28454322
UR  - PM:28454322
N1  - PMID:28454322
ID  - Kim_etal2017
ER  -