TY - JOUR AU - Kim, M.Y. AU - Park, S.-J. AU - Shim, J.W. AU - Song, Y.J. AU - Yang, K. AU - Heo, K. PY - 2017// TI - Accumulation of low-dose BIX01294 promotes metastatic potential of U251 glioblastoma cells T2 - Oncol Lett JO - Oncology Letters SP - 1767 EP - 1774 VL - 13 IS - 3 KW - Bix01294 KW - epithelial-mesenchymal transition KW - glioblastoma stem cells KW - metastasis AB - BIX01294 (Bix) is known to be a euchromatic histone-lysine N-methyltransferase 2 inhibitor and treatment with Bix suppresses cancer cell survival and proliferation. In the present study, it was observed that sequential treatment with low-dose Bix notably increases glioblastoma cell migration and metastasis. It was demonstrated that U251 cells sequentially treated with low-dose Bix exhibited induced characteristic changes in critical epithelial-mesenchymal transition (EMT) markers, including E-cadherin, N-cadherin, beta-catenin and zinc finger protein SNAI2. Notably, sequential treatment with Bix also increased the expression of cancer stem cell-associated markers, including sex determining region Y-box 2, octamer-binding transcription factor 4 and cluster of differentiation 133. Neurosphere formation was significantly enhanced in cells sequentially treated with Bix, compared with control cells (control: P=0.011; single treatment of Bix, P=0.045). The results of the present study suggest that accumulation of low-dose Bix enhanced the migration and metastatic potential of glioblastoma cells by regulating EMT-associated gene expression, which may be the cause of the altered properties of glioblastoma stem cells. SN - 1792-1074 UR - http://www.ncbi.nlm.nih.gov/pubmed/28454322 UR - PM:28454322 N1 - PMID:28454322 ID - Kim_etal2017 ER -