%0 Journal Article
%T Percentage of mesenchymal stem cells in high-grade glioma tumor samples correlates with patient survival
%A Shahar, T.
%A Rozovski, U.
%A Hess, K.R.
%A Hossain, A.
%A Gumin, J.
%A Gao, F.
%A Fuller, G.N.
%A Goodman, L.
%A Sulman, E.P.
%A Lang, F.F.
%J Neuro-Oncology
%D 2017
%V 19
%N 5
%@ 1522-8517
%F Shahar_etal2017
%O PMID:28453745
%O exported from refbase (http://demo.refbase.net/show.php?record=96589), last updated on Fri, 20 Oct 2017 10:21:27 +0200
%X Background: Human mesenchymal stem cells (hMSCs) have been shown to reside as stromal cells in human gliomas as glioma-associated hMSCs (GA-hMSCs), but their biological role remains unclear. Because recent evidence indicates that GA-hMSCs drive tumor cell proliferation and stemness, we hypothesized that a higher percentage of GA-hMSCs in tumors predicts poor patient prognosis. Method: We determined the percentage of cells coexpressing GA-hMSC markers CD105+/CD73+/CD90+ from patients with newly diagnosed high-grade glioma and analyzed the association between this percentage and overall survival (OS) in 3 independent cohorts: fresh surgical glioblastoma specimens (cohort 1, N = 9), cultured tumor specimens at passage 3 (cohort 2, N = 28), and The Cancer Genome Atlas (TCGA) database. Results: In all cohorts, patient OS correlated with the percentages of GA-hMSCs in tumors. For cohort 1, the median OS of patients with tumors with a low percentage of triple-positive cells was 46 months, and for tumors with a high percentage of triple-positive cells, it was 12 months (hazard ratio [HR] = 0.24; 95% CI: 0.02-0.5, P = .02). For cohort 2, the median OS of patients with tumors with a low percentage of GA-hMSCs was 66 months, and for tumors with a high percentage, it was 11 months (HR = 0.38; 95% CI: 0.13-0.9, P = .04). In the database of TCGA, the median OS times in patients with high and low coexpression levels of CD105/CD73/CD90 were 8.4 months and 13.1 months (HR = 0.4; 95% CI: 0.1-0.88; P = .04), respectively. Conclusions: The percentage of GA-MSCs inversely correlates with OS, suggesting a role for GA-MSCs in promoting aggressive behavior of gliomas.
%K *glioblastoma
%K *mesenchymal stem cells
%K *microenvironment
%K *prognosis
%U http://www.ncbi.nlm.nih.gov/pubmed/28453745
%P 660-668